《植物生理学报》 2010, 46(11): 1164-1168

培养条件对澳蜡花‘ 舞后’ 试管苗玻璃化的影响

孟会, 李青*, 潘会堂

北京林业大学园林学院, 国家花卉工程技术中心, 北京 100083

通信作者：李青；E-mail: wliqing06@sina.com；Tel: 010-62338294

摘　要：

以澳蜡花‘ 舞后’ 的试管苗为试验材料, 研究了培养条件对其试管苗玻璃化的影响。结果表明, 当以1/2MS为基本培养基, 附加0.2 mg·L-1 6-BA、0.01 mg·L-1 IBA、4% 蔗糖、0.8%琼脂并在以塑料封口膜为封口材料的三角瓶内培养时, 试管苗的增殖系数较高, 玻璃化率较低。

关键词：澳蜡花; 试管苗; 玻璃化

收稿：2010-06-04   修定：2010-08-18

资助：国家林业局“948” 项目(2007-4-07)。

Effects of Tissue Culture Conditions on Vitrification of in vitro shoots of Chamelaucium uncinatum Schauer ‘Dancing Queen’

MENG Hui, LI Qing*, PAN Hui-Tang

National Floriculture Engineering Research Center, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Corresponding author: LI Qing; E-mail: wliqing06@sina.com; Tel: 010-62338294

Abstract:

Chamelaucium uncinatum ‘Dancing Queen’ were chosen as materials to study the effects of different conditions of tissue culture on vitrification. Results showed the optimum medium for proliferation and overcoming vitrification was 1/2MS medium supplemented with 0.2 mg·L^-1 6-BA, 0.01 mg·L^-1 IBA, 4% sugar and 0.8% agar, using conical flask closed with good permeability sealing material.

Key words: Chamelaucium uncinatum; in vitro shoots; vitrification